Analysis Of Phyllantus Niruri (Ewe Olobe) Cold Water Leaf Extract For The Control Of Some Selected Fungi

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ABSTRACT

A laboratory test was conducted using cold water extract of leaves of phyllanthus niruri on some selected fungi such as aspergillus niger, aspergillus flavus, Rphizops spp, penicillium spp and mucor spp respectively. The result of the experiment shows that the extract has some inhibitory activities on the selected fungi. This shows that the extract has fungicidal properties and hence can be used in the treatment of some fungal disease (infections).

TABLE OF CONTENTS

Title page

Certification

Dedication

Acknowledgements

Table of contents

Abstract

CHAPTER ONE

1.1 Introduction and literature review


CHAPTER TWO

2.1 Materials and Methods

2.2 sterilization of Materials

2.3 Culture Media Preparation

2.4 Collection of Plant Materials

2.5 Method of Extraction

2.6 Sampling Procedure

2.7 Isolation of Pure Culture

2.8 Antifungal Test

CHAPTER THREE

3.0 Result

3.1 Fungi colony counts

3.2 Cultural characteristics and morphology of isolated fungi

3.21 Aspergillus niger

3.2.2  Penicillium spp

3.2.3  Phizopus spp

3.2.4  Aspergillus flavus

3.2.5  Mucor spp

3.3.Antifungal activity of phyllanthus niruri leave extract on the fungi isolates.

CHAPTER FOUR

4.1 Discussion

4.2Conclusion and Recommendation

4.3 References

CHAPTER ONE

1.0 INTRODUCTION

Phyllanthus niruri is a widespread tropical plant commonly found in coastal areas; best known by the common mane stone-breaker or seed-under leaf. It is a relative of the spurges belongs to the Phyllanthus genus of family Euphorbiaceae, species; Niruri and amarus.

The synonyms of Phyllanthus niruri include Phyllanthus carolianus, sellowianus and Phyllanthus lonphali. The common names for Phyllanthus niruri include chanca piedra in Spanish, phumyamalaki in ayurveda and Quebra Pedra in Portuguese (Keelanelli, 2013).

The Phyllanthus niruri is also known in Yoruba Language as “EWE OLOBE”. Phyllanthus niruri originated in India usually occurring as a winter weed throughout the hotter parts. The Phyllanthus genus contains over 600 species of shrubs, trees and annual or biennial herbs distributed throughout the tropical and sub-tropical areas.

The Phyllanthus niruri is a common Kharif (rainy season) weed found in both cultivated fields and wastelands. Recently it ahs attracted the attention of researchers because of its hepatoprotective properties. No effective specific therapy is available for viral hepatitis but Phyllanthus niruri has shown clinical efficacy in viral hepatitis B [Keelanelli, 2013.]

 

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